The drama - starring Zoe Lucker and Ben Price - aired on ITV from January 2002 to April 2006.
Smith's sister Laura Jayne told The Daily Mail: "I had a friend who worked on Footballers' Wives so I covered Matt in fake tan, gelled his hair, made him wear salmon pink trousers and sent him for an audition."
However, Smith - who won the role of the Doctor in 2009 - failed to impress the Footballers' Wives team.
Prior to replacing David Tennant on Doctor Who, Smith also auditioned to play John Watson in BBC drama Sherlock, a role eventually won by Martin Freeman.
Doctor Who returns to BBC One in the autumn.
> Doctor Who Steven Moffat: 'Matt Smith hasn't decided when he'll go'
Watch the trailer for the next series of Doctor Who below: