The video shows Spider-Man at odds with Oscorp, introducing new villain the Iguana.
The Iguana in The Amazing Spider-Man will differ from the version that debuted in comics back in 1979. As the product of genetic experiments gone wrong, the seven foot monster is more animal than human, making the Iguana a dangerous enemy in the game.
The Amazing Spider-Man is launching this summer to coincide with the cinematic release of the movie of the same name, starring Andrew Garfield, Emma Stone and Rhys Ifans.
Beenox Studios, the team behind Spider-Man: Shattered Dimensions and Spider-Man: Edge of Time, are handling the project's development. Its debut trailer was first shown at the Spike Video Game Awards in December 2011.
The Amazing Spider-Man movie will be released on 3D, IMAX and conventional screens on July 3 worldwide. The game will be available for Xbox 360, PlayStation 3, Wii, 3DS and DS on June 26.
Scroll through the gallery of Iguana images from The Amazing Spider-Man game below: