The latest video, which was unveiled at Capcom's Captivate event, confirms a new release date of October 2.
The plot-heavy trailer also examines the origins of the Javo, which were created using the C-Virus.
The identity of the "mysterious third protagonist" is revealed, while Sherry Birkin and Ada Wong also make an appearance.
The dramatic video ends with a stand-off between Chris Redfield and Leon Kennedy, before the new release date is confirmed.
Announced earlier this year, Resident Evil 6 will be available on Xbox 360 and PlayStation 3. A PC release will follow, but has not yet been dated.
The upcoming sequel is set 15 years after the Raccoon City incident and takes place in the infected town of Tall Oaks, as well as China.
The game is Capcom's largest ever production, with more than 600 staff involved in its development.
Watch the Resident Evil 6 announcement trailer below: