The 2D fighting game will be available to download from Steam, Origin, GameFly, GameStop PC Downloads and GamersGate this autumn.
The PC version adds in-game move-lists, additional tutorials, improved single-player AI, enhanced leaderboards and online fixes, which include multi-region battles and name displays. The updates will be rolled out to console users in the coming months.
The news comes on the same day that Skullgirls launches for the PlayStation 3 on PSN.
More details about the game's plot can be found by watching the recently-released Story trailer.
The downloadable title is developed by Reverge Labs and published by Def Jam Rapstar studio Autumn Games. It aims to address many common complaints within the fighting genre.
An extensive tutorial mode and relaxed input timings for complicated moves aim to make it more accessible for newcomers.
Meanwhile, a ratio tag battle system means players can opt to fight with a single character that has the same HP as the opponent.
Skullgirls is also one of the first dedicated console titles to make use of the GGPO (Good Game Peace Out) netcode for online play.
The GGPO system aims to eliminate latency issues that have plagued online arcade fighting games. It is also used in Street Fighter III: Third Strike Online Edition.
Watch the Skullgirls launch trailer below: