Teased yesterday, the video sees a battered Agent 47 fight an ambush from a deadly wetworks team known as 'The Saints'.
The first new Hitman game in six years, Hitman: Absolution will include new gameplay features, improved enemy intelligence and a new 'Instinct' mode that will help players blend into certain roles.
Described as the most ambitious game in the series yet, the stealth title features a "living and breathing world, where every moment can become a story".
IO Interactive told Digital Spy that Hitman Absolution will have a "more personal story" than previous games.
Pre-order mini-game Hitman Sniper Challenge was released earlier this month.
The E3 Gaming Expo will run from June 4 to 7.
Hitman Absolution will be available on Xbox 360, PS3 and PC from November 20 worldwide.
Watch a gameplay trailer for Hitman Absolution below: