The announcement is accompanied by a new trailer from the Tokyo Game Show, featuring the likes of Batman, Harley Quinn, Superman, The Flash, Wonder Woman and Catwoman.
The latest video showcases the DC-based brawler's extensive move set, combo system, super moves and background damage.
Announced in May, Injustice: Gods Among Us is said to have a deep original story, taking place in "a world where the lines between good and evil are blurred".
NetherRealm is developing the title following last year's success with Mortal Kombat. In 2008, the team also created Mortal Kombat vs DC Universe as Midway Games.
The developers have dropped the traditional three-round fighting system, opting instead for a "more cinematic" single-round system.
Injustice: Gods Among Us will be available on Xbox 360, PlayStation 3 and Wii U.
Watch the Injustice: Gods Among Us announcement trailer below: