MGM and 20th Century Fox confirmed at the 2012 Consumer Electronics Show that the collection will include all 22 films so far, nine of which have never been released on Blu-ray, and over 130 hours of bonus features.
"With all 22 feature films available on Blu-ray in one collection for the first time this is a great way for fans to catch up on 007's epic journey before Skyfall hits theaters next fall," Michael Brown, senior vice president of MGM Home Entertainment, commented.
"Now viewers can enjoy the intense action of the innovative franchise in the most immersive home experience possible."
Bond executives Michael G Wilson and Barbara Broccoli said that the boxset announcement marks the beginning of "a whole programme of exciting activities" for the 50th anniversary.
The pack artwork has since been unveiled on the official Bond Facebook page.
Wilson previously confirmed plans to reunite all six Bond actors - Sean Connery, George Lazenby, Roger Moore, Timothy Dalton, Pierce Brosnan and Daniel Craig - for a 50th anniversary celebration.
"We're trying to find a way for fans to celebrate with us because they've been the reason the Bond films have been the success they have," he said.
The 23rd Bond film Skyfall - which is Craig's third outing as the legendary spy - is currently filming in the UK.
The Sam Mendes-directed Skyfall will arrive in cinemas on October 26, 2012.
Watch the trailer for the James Bond Blu-ray boxset below: