The footage shows Jason Biggs, Alyson Hannigan, Seann William Scott and Eugene Levy reprising their roles from the original American Pie trilogy.
Tara Reid, Eddie Kaye Thomas, Thomas Ian Nicholas and Mena Suvari are also among the returning cast for the fourth cinematic entry in the comedy series.
American Reunion sees the original gang organising a ten-year reunion through Facebook, with the group discovering how their lives have changed since the events of 2003's American Wedding.
Scott previously told Digital Spy that the idea to reunite the American Pie characters came about from a pitch he made three years previous.
American Reunion arrives in US cinemas on April 6, followed by a UK launch on May 2.
> American Reunion: Watch the new red band trailer
> Jason Biggs, Tara Reid in American Reunion photobooth trailer
> American Reunion first teaser poster released
Move through our American Reunion picture gallery below: