Last week, it was claimed that a film about Hendrix will start shooting in Ireland this month with OutKast rapper Andre 3000 portraying the iconic musician.
However, the Hendrix estate released a statement - echoing almost entirely a previous release on the subject in 2011 - that it "has made it known many times in the past that no such film, were it to include original music or copyrights created by Jimi Hendrix, can be undertaken without its full participation".
The estate added that it is not opposed to projects about Hendrix as long as they are approved by the family.
"Experience Hendrix CEO Janie Hendrix, sister of Jimi Hendrix, and the EH board have not ruled out a 'biopic' in the future though producing partners would, out of necessity, have to involve the company from the inception of any such film project if it is to include original Jimi Hendrix music or compositions," the statement also said.
A Jimi Hendrix biopic has been rumoured for years, with several celebrities linked to the lead role, such as Laurence Fishburne, Lenny Kravitz and 'Whatcha Say' singer Jason Derulo.
Watch Jimi Hendrix perform 'Hey Joe' below: