In the 'Origins' clip, the cast and crew talk about the film's main themes, and how they tie into real-world scientific theory and fact.
"The Catholic church, NASA and various scientists say, without any question, there is biology out there in this galaxy," director Ridley Scott says in the video.
Prometheus centres around a team of explorers who go on a mission to find the origins of mankind on Earth, before finding themselves in a battle to save the entire human race.
The project stars Noomi Rapace, Logan Marshall-Green, Michael Fassbender, Idris Elba, Guy Pearce and Charlize Theron.
Co-writer Damon Lindelof reveals: "Ridley was very interested in, 'What if the origin of life on earth was basically seeded by extra-terrestrials?'"
On the film's real-world scientific base, Scott continues: "This is fundamentally based on feasibility. This story walks around the truth, of what may be out there.
"They are on the search for what they believe may have created human beings. How much do we really know? Instead of a fiction, what if it's a reality? It presents big questions."
He adds: "Of course, what I want to do is scare the living s**t out of you."
Prometheus opens to cinemas next month on June 1 in the UK and June 8 in the US.
Prometheus in pictures - Photo gallery: