According to The Hollywood Reporter, the total box office gross for the superhero film rose to $1.33bn (£866m) yesterday, surpassing the $1.328bn (£864m) total of Harry Potter and the Deathly Hallows: Part 2.
Still ahead of The Avengers on the all-time list are Titanic, at number two with $1.8bn (£1.2bn), and Avatar, at number one with $2.8bn (£1.8bn).
The Avengers also became the third-highest grossing film ever in the US, overtaking The Dark Knight with cumulative domestic earnings of $538.1m (£350m).
Iron Man 3, the next film in the Avengers series, is currently in production for a Summer 2013 release.
A direct sequel to The Avengers is currently in development, although Whedon is yet to confirm his involvement as director.
Watch a clip from The Avengers below: