The band, who were celebrating guitarist Jamie Cook's 26th birthday, were also joined on the Main Stage by Miles Kane for the finale of their set, finishing with the track '505' from 2007's Favourite Worst Nightmare.
"It's Jamie Cook's birthday today everybody, so anytime it goes quiet at all this evening, you just start singing 'Happy Birthday'," frontman Alex Turner said at the beginning of the set, before leading the crowd in singing the song to Cook.
Later, as he introduced Kane to the stage, Turner said:"Not only is he our best friend, but our best dressed friend."
Prior to the show, drummer Matt Helders told NME that having four albums meant the band could do a "good mixture" of songs.
Kane recently supported Arctic Monkeys at the iTunes Festival.
> Read our review of Arctic Monkeys' new album Suck It and See
Arctic Monkeys' full setlist below:
'This House Is A Circus'
'Still Take You Home'
'Don't Sit Down 'Cos I've Moved Your Chair'
'Brick By Brick'
'The Hellcat Spangled Shalala'
'The View From The Afternoon'
'I Bet You Look Good On The Dancefloor'
'Suck It And See'
'If You Were There, Beware'
'Do Me A Favour'
'When The Sun Goes Down'
Watch Arctic Monkey's performing '505' with Miles Kane at T in the Park 2011 below: