Madonna's chat with Fallon will be streamed on her official Facebook page and will be her first live interview in promotion of her new album MDNA.
The 'Girl Gone Wild' singer will also take fan questions about MDNA and her upcoming world tour, which kicks off on May 29 in Tel Aviv.
Madonna's live Facebook interview will stream at 6/5c on Saturday, March 24.
MDNA leaked online on Tuesday (March 20), a full week ahead of its official release on March 26.
The popstar had been officially releasing snippets of the 17-track deluxe version of MDNA on Facebook prior to the leak.
MDNA is Madonna's 12th studio album and the follow-up to 2008's global chart-topper Hard Candy.
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Watch Madonna's 'Girl Gone Wild' music video below: