The boyband were in the recording studio with the Canadian superstar working on a new track earlier this week.
One Direction's Niall Horan tweeted: "in the studio with our boy @justinbieber last night , its gona sound great! (sic)"
The news follows claims by Bieber's manager Scooter Braun that the collaboration should happen because "all people of that generation should be there to help each other".
One Direction recently became the first British group to top the US Billboard 200 with their debut album Up All Night.
The boyband have since announced a special headline show at New York's Madison Square Garden on December 3.
Meanwhile, Bieber's latest single 'Boyfriend' shifted over 520,000 copies in the US during its first week of release.
> Justin Bieber: 'One of world's biggest artists fancies Harry Styles'
Listen to Justin Bieber's 'Boyfriend' below: