'Honeycomb' and 'Gotham', a double A-side single, will be available as a download and on vinyl.
The tracks will be released on June 26 from Domino Records.
The single follows their recent Record Store Day release 'Transverse Temporal Gyrus'.
Animal Collective released the well-received Merriweather Post Pavilion in 2009, before the experimental visual collection ODDSAC in 2010.
Frontman Panda Bear hinted in early 2010 that Animal Collective may have come to an end.
Listen to the tracks below: