The boyband performed an acoustic rendition of their global hit 'What Makes You Beautiful' as part of a VEVO Go session.
The group surprised fans in a diner who thought they were at the venue to watch a preview of their new tour DVD Up All Night: The Live Tour.
The largely female crowd sang along with the five-piece, while members Louis Tomlinson and Liam Payne used tomato ketchup bottles as microphones at one point.
One Direction are currently travelling the US as part of their first ever headline jaunt across the country.
The group's Up All Night: The Live Tour DVD will be released on May 28.
Earlier this week, Professor Green performed the first ever VEVO Go session in the UK on London's Carnaby Street.
Watch One Direction's VEVO Go session below: