The 'Lollipop' rapper is suing Quincy Jones III for alleged copyright infringement, according to TMZ.
He claims that Jones illegally used music from his platinum-selling album Tha Carter IV in an unauthorised documentary about his life.
The Grammy winner is now demanding unspecified damages as well as a court injunction that would ban all producers from using his music in the future.
This is the second lawsuit Lil Wayne has launched in regards to documentary The Carter.
In 2009, he sued Jones's production company QD3 Entertainment for fraud and breach of contract, after the makers of the film allegedly backed out on a deal to give the rapper final approval.
Lil Wayne recently refused to pay a $400,000 fine in a court judgement regarding a breach of contract with a concert promoter.