Palfrey and Kemp Fords in Lymington, Hampshire, is reportedly famed for its owners Terry Palfrey and Geoff Kemp's aggressive dealings with customers.
The pair have shouted and chucked out customers on several occasions during their time at the store.
The store space has been used for trading purposes for 170 years, and will now be replaced by a Wetherspoon's pub.
A sign in the shop window reads: "Britain's Rudest Shop is Closing Down.
"After 170 years only a few weeks left to be insulted, don't miss the experience!!"
Palfrey, 63, told the Southern Daily Echo: "I have chucked people out of the shop on several occasions.
"Sometimes the other customers even clap because they enjoy it. Unfortunately there are a lot of people these days who don't have any manners.
"If it gets too bad, I'll tell them to get out - I just will not stand for it."
Palfrey added that he particularly hated customers who fiddled with packed bed linen or left finger marks on polished cutlery.
The original proposal to replace the shop with a pub chain saw over 900 complaints from local residents.