Molecule, a water-only cafe in East Village, prices tap water at a substantial $2.50 (£1.50), reports Metro.
Most Americans can expect to receive free tap water upon request at restaurants.
However, Molecule's owners pointed out that the establishment's tap water is filtered through a $20,000 (£12,000) machine.
Co-owner Adam Ruhf said: "It's about treating water a little more consciously, mindfully and respectfully."
For an extra charge, waiters also offer to sweeten up the customer's beverage with vitamins.
"Our supplements are based on medicinal herbs and roots from around the world," Ruhf added.
The company plans to diversify the business by bottling the tap water and delivering it.