During an interview on Sunday Night, the singer's bump appeared to collapse inwardly as she sits down on a chair.
Rumors then began circulating online that the 'Countdown' star has enlisted a surrogate mother and is actually wearing a fake prosthetic baby bump.
However, Beyoncé's publicist Yvette Noel-Schure told E! News that the speculation is "foolish".
Beyoncé was accused of wearing a fake baby bump while attending the MTV VMAs earlier this year.
During her interview on Sunday Night, the star revealed that her baby is due in February.
> Beyoncé: 'Pregnancy is an incredible gift'