The singer beat competition from the likes of Sofia Vergara, Charlize Theron, Michelle Williams and Kate Middleton to top the list, which was compiled by People magazine.
"I feel more beautiful than I've ever felt because I've given birth," Beyoncé said. "I have never felt so connected, never felt like I had such a purpose on this earth."
Speaking further about her daughter Blue Ivy Carter, Beyoncé added: "She's just the cutest thing."
Asked if her daughter resembles her mother or her father Jay-Z more, the star responded: "She looks like Blue. She's her own person.
"The best thing about having a daughter is having a true legacy. The word 'love' means something completely different now."
Beyoncé recently Skype called Polish producer Jimek, who won a remix competition after re-working the singer's new single 'End Of Time'.
> Beyoncé: 'Michelle Obama is a role model for Blue Ivy'
Watch behind-the-scenes footage of Beyoncé's 'World's Most Beautiful Woman' shoot below:
Photo gallery - view People's Most Beautiful Women in pictures: