The duo are to join The Daily Show's Jon Stewart and artist Cindy Sherman to present a video tribute to the late Jobs.
Former US President Bill Clinton and ex-Vice President Al Gore will share their memories of Jobs as well, according to Page Six.
Jobs passed away last October at age 56 from pancreatic cancer, drawing remembrances on social media from US President Barack Obama, Bill Gates, Mark Zuckerberg, Ashton Kutcher and Arnold Schwarzenegger.
This year's Webby Awards are held at the Hammerstein Ballroom in New York City on May 21.
Young Adult actor Patton Oswalt is to host the gala.