All funds raised from auctioning the limited edition model of the electric car will help fund the Satellite Sentinel Project, which uses cameras to monitor violence in the region.
(George Clooney is pictured above with his girlfriend Stacy Keibler at the 2012 Academy Awards)
The public sale - which is expected to raise at least $100,000 - is to be held at the Pebble Beach Concours d'Elegance in California on August 18.
David Gooding of the Gooding & Co auction house said in a statement that his firm is delighted to be aiding such a worthy cause.
"We are overjoyed to present George Clooney's Signature 100 Tesla at Pebble Beach because this is an incredible opportunity to purchase a modern collector car with all the right elements - one-owner, celebrity provenance; historical significance; limited-edition rarity and philanthropic tie," Gooding said.
Clooney launched the Satellite Sentinel Project in December 2010 as part of his Not On Our Watch campaign. He has been joined by Brad Pitt and Matt Damon in funding the project.
The Oscar winner was arrested in Washington DC earlier this year for attending a protest at Sudan's US embassy.
Watch footage of George Clooney's Sudan protest arrest below: