Diane, played by Alex Fletcher, discovers that she is pregnant later this month after her recent nights of passion with Tony Hutchinson (Nick Pickard).
Regular viewers will know that Diane has always desperately wanted a baby of her own, but it won't be plain sailing for her as she is unsure how much support she can expect from Tony.
A show source told Digital Spy: "Diane knows that her fling with Tony wasn't anything serious and his heart has always belonged to Jacqui McQueen.
"At first she's reluctant to tell Tony about the baby, but she finally plucks up the courage to let him know that he's going to be a father again.
"With Jacqui bowing out from the village soon, there may still be a chance of Tony and Diane becoming a proper couple, but viewers will have to wait and see how it pans out."
Another major obstacle for the pair will be Tony's upcoming battle with testicular cancer. As previously revealed, the businessman will be diagnosed with the illness in early May.
The insider added: "With a baby on the way and serious health problems to worry about, Tony is going to have a lot on his plate. There are interesting times ahead for him and Diane."
Diane's early months on Hollyoaks in 2010 saw her undergoing IVF treatment, as she hoped for a child of her own after raising stepkids Sinead and Finn.
More recently, Diane has agreed to adopt Sinead's baby girl when she arrives later this year.
Hollyoaks airs weeknights at 6.30pm on Channel 4, with first look screenings at 7pm on E4.
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