The 85-year-old actress - who is currently undertaking a six-month break from the soap as Dot Cotton - features on her godson Tim Arnold's track 'Soho Heroes', reports The Mirror.
Arnold - who is dating Brown's co-star Jessie Wallace - records under the stage name Soho Hobo.
The track is described as "somewhere between Blur and the Divine Comedy" and is released on Monday (July 23).
A source said: "June and Tim are great friends.
"She loves to help him out. She loves his music and Tim is delighted about her agreeing to take part."
The song is dubbed as a "tribute to the figures who have each made their mark living or working in Soho over 400 years".
Brown recently made a tearful reunion with her former co-star John Bardon for a BBC TV documentary.
> EastEnders' June Brown visits John Bardon 'regularly'
> Jessie Wallace's ex-fiancé Vince Morse gives blessing to Tim Arnold
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