Kuruma de DS, recently showcased at a Tokyo press conference, consists of a DS cartridge that interacts with the car manufacturer's Smart Navi system via Bluetooth.
Drivers can their use the gaming system to view maps, edit destinations, and check their speed, Kotaku understands.
Toyota's Smart Navi system is priced at ¥206,850 (£1,616), while the DS cartridge costs ¥7,329 (£56). The technology is expected to be exclusive to Japan.
Nintendo's 3DS hit the milestone of 5 million units sold in February this year, making it the fastest system to hit the landmark figure in the territory.
Kuruma de DS launches in Japan on June 1.