The drama pilot will follow Susannah (Tricia Helfer), a K-9 Search and Rescue volunteer who solves crimes with her golden retriever Puzzle.
Glau will play a beautiful but tough member of Susannah's team, according to Deadline.
The actress previously starred in short-lived NBC drama The Cape and also appeared in Fox shows Firefly and Terminator: The Sarah Connor Chronicles.
Brothers actor Daryl Mitchell will also appear in Scent of the Missing as the team's wheelchair-bound communications technician.
Mitchell starred in NBC comedy-drama Ed from 2002 to 2004 and recently played guest roles on Desperate Housewives and Traffic Light.
Production on the Scent of the Missing pilot, based on the book by Susannah Charleson, is expected to begin in late November.
CSI's Carol Mendelsohn has co-written the project and will also executive produce alongside Melissa R Byer, Treena Hancock, Barry Josephson and Julie Weitz. CBS Television Studios is producing.
> Eric Winter joins TNT pilot Scent of the Missing