Chris O'Donnell's mother Julie and Daniela Ruah's parent Catarina will make cameo appearances in the series, reports TVLine.
However, they will not be starring as the actors' on-screen mothers, as they have already been introduced on the CBS drama.
They will be joined by LL Cool J and Eric Christian Olsen's mothers as a group of gambling women at a casino.
CBS has said that they will feature in a scene "hand-picked by executive producer Shane Brennan."
NCIS: Los Angeles will return for a fourth season on Tuesday, September 25 on CBS.
The action series was picked up for a fourth season back in March.