The actress, who plays Gabrielle Solis, revealed that the 2004 pilot of Marc Cherry's series is the most meaningful to her.
"My favourite episode of all time is still the pilot," she told The Huffington Post. "There was an innocence in filming that. We didn't really have any expectations and we didn't know what to expect."
Asked what other Housewives episodes stand out in her memory, she said: "I love the 100th episode with Beau Bridges... that was one of my favourite episodes. And season eight has been so good, so there's a lot of memories on this season."
Longoria previously expressed hope that the final episode of Desperate Housewives is "a little open-ended", but said that she does not want a movie adaptation.
Desperate Housewives returns to ABC on April 29.
> 'Desperate Housewives' Marc Cherry promises "satisfying" final episode
Watch the opening of the Desperate Housewives pilot below: