The explosive video features brief clips of cast members including Anna Paquin, Stephen Moyer and Alexander Skarsgård.
It also confirms that the next season of True Blood will premiere on HBO in June. Previous trailers had simply listed its return date as summer.
Law & Order: SVU star Christopher Meloni, Spartacus actor Peter Mensah and Camilla Luddington of Californication are among the new additions to the cast.
True Blood showrunner Alan Ball has confirmed that he will step down after this season.
"True Blood has been, and will continue to be, a highlight of not only my career but my life," he said in February. "The show will continue to thrive as I look forward to new and exciting ventures."
> 'True Blood' season five: Three new promo videos released